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为探讨淋巴细胞表面Ｆａｓ及其配体（ＦａｓＬ）在ＩＬ－２和ＩＬ－１２作用下表达的变化，以及Ｆａｓ和ＦａｓＬ与淋巴细胞抗肿瘤活性的关系，作者用免疫荧光标记和流式细胞仪分析了ＩＬ－２和ＩＬ－１２激活的杀伤细胞（ＩＬ－２ＡＫ和ＩＬ－１２ＡＫ）Ｆａｓ和ＦａｓＬ的表达；分别用乳酸脱氢酶（ＬＤＨ）释放法和ａｎｎｅｘｉｎ－Ｖ－ＦＩＴＣ标记检测ＩＬ－２ＡＫ和ＩＬ－１２ＡＫ杀伤的肿瘤细胞的坏死和凋亡。结果表明：①正常人外周血淋巴细胞（ＰＢＬ）（２０．４０±５．６９）％表达Ｆａｓ，但无ＦａｓＬ表达；３株消化道肿瘤细胞系恒定表达Ｆａｓ，始终不表达ＦａｓＬ；②ＩＬ－２与ＩＬ－１２可诱导ＬＡＫ细胞表达ＦａｓＬ（Ｐ＜０．０１），并促使Ｆａｓ表达显著增加（Ｐ＜０．０１）；③ＬＡＫ细胞致肿瘤细胞死亡中ＦａｓＬ表达与ａｎｎｅｘｉｎ－Ｖ标记阳性率和肿瘤细胞表面Ｆａｓ表达正相关。由此推论，Ｆａｓ－ＦａｓＬ途径是ＩＬ－２与ＩＬ－１２诱导活化的ＬＡＫ细胞杀伤肿瘤的途径之一，淋巴细胞致肿瘤凋亡分别与淋巴细胞ＦａｓＬ表达和肿瘤细胞Ｆａｓ表达一致。
To investigate the changes of the expression of Fas and its ligand (FasL) on the surface of lymphocytes under the action of IL-2 and IL-12, and the relationship between Fas and FasL and the anti-tumor activity of lymphocytes, the authors used immunofluorescence labeling and flow cytometry The expressions of Fas and FasL in IL-2 and IL-12 activated killer cells (IL-2AK and IL-12AK) were analyzed. The expression of IL-2AK was detected by lactate dehydrogenase (LDH) release assay and annexin-V- And tumor necrosis and apoptosis of IL-12AK-killed tumor cells. The results showed that: (1) normal human peripheral blood lymphocytes (PBL) expressed Fas but no FasL expression; 3 of the gastrointestinal cancer cell lines constantly expressed Fas and never expressed FasL; ②IL-2 And IL-12 induced FasL expression in LAK cells (P <0.01), and promoted the expression of Fas significantly (P <0.01). (3) FasL expression was positively correlated with the positive rates of annexin-V in tumor cells Fas positive cell surface expression. It is concluded that Fas-FasL pathway is one of the pathways of killing tumor cells induced by IL-2 and IL-12-activated LAK cells. Lymphocyte-induced apoptosis is consistent with FasL expression of lymphocytes and Fas expression of tumor cells, respectively.