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目的：探讨急性重型肝炎患者肝组织中庚型肝炎病毒（ＨＧＶ）的存在状况及其与发病的关系；方法：采用免疫组化方法以抗—ＨＧＶＮＳ５单克隆抗体对２７例急性重型肝炎患者尸检肝组织中的ＨＧＶ等抗原进行了检测；结果：２７例肝组织中检测出ＨＧＶＮＳ５阳性６例（２２．２％）。ＨＧＶＮＳ５Ａｇ阳性着色颗粒表达于残存的肝细胞胞浆内，阳性细胞呈片簇状分布于汇管区周围。６例中４例重叠有ＨＢＶ或／和ＨＣＶ感染，肝组织中ＨＢｓＡｇ或／和ＨＣＶＮＳ５Ａｇ检测阳性，另２例肝组织中仅见ＨＧＶＮＳ５抗原表达，回顾病史，其中１例于住院期间接受过输血，住院后次日死去，另１例入院后不久死去，未输用任何血制品；结论：ＨＧＶ感染可能与急性重型肝炎发病有关
Objective: To investigate the presence of hepatomegaly hepatitis virus (HGV) in liver tissue of patients with acute severe hepatitis and its relationship with the pathogenesis.Methods: Anti-HGVNS5 monoclonal antibody was used in 27 patients with acute severe hepatitis HGV and other antigens in tissues were detected. Results: 6 cases (22.2%) of HGVNS5 positive were detected in 27 cases of liver tissues. HGVNS5Ag positive staining particles expressed in the remaining cytoplasm of hepatocytes, positive cells were clustered around the portal area. Of the 6 cases, 4 cases were found to have HBV or HCV infection and HCV and HBsAg positive or / and HCV NS5Ag positive in liver tissue. Only 2 cases of HGVNS5 antigen expression were observed in liver tissues. One case was hospitalized with transfusion and hospitalization Died on the next day, another died shortly after admission, did not lose any blood products; Conclusion: HGV infection may be related to the incidence of acute severe hepatitis