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AIM:To study the polymorphism of flagellin A genotype andIts significance in Helicobecter pylori(H.pylori).METHODS:As the template,genome DNA was purified fromsix clinical isolates of H.pylori from outpatients,and thecorresponding flagellion A fragments were amplified bypolymerase chain reaction.All these products weresequenced.These sequences were compared with eachother,and analyzed by software of FASTA program.RESULTS:Spaciflc PCR products were amplified from all ofthese H.pylorl isolates and no length divergence wasfound among them.Compared with each other,the highestungappad identity is 99.10%,while the lowest is 94.65%.Using FASTA program,the alignments between query andllbary sequences derived from different H.pylori strainswere higher than 90%.CONCLUSION:The nucleotide sequence of flagellin A in H.pylori is highly conservative with Incident divergence.ThisInformation may be useful for gene diagnosis and furtherstudy on flagellar antigen phenotype.
AIM: To study the polymorphism of flagellin A genotype and importance significance in Helicobecter pylori (.pylori) .METHODS: As the template, genome DNA was purified fromsix clinical isolates of H.pylori from outpatients, and thecorresponding flagellion A fragments were amplified by polymerase chain reaction.All these products were sequenced. These sequences were compared with eachother, and analyzed by software of FASTA program .RESULTS: Spaciflc PCR products were amplified from all of these H.pylorl isolates and no length divergence wasfound among them. Compared with each other, the The highestungappad identity is 99.10%, while the lowest is 94.65%. Using the FASTA program, the alignments between query and information derived from different H. pylori strains greater than 90% .CONCLUSION: The nucleotide sequence of flagellin A in H. pylori is highly conservative with Incident divergence. This information may be useful for gene diagnosis and furtherstudy on flagellar antigen phenotype.