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采用扫描电镜(SEM)、傅立叶变换红外光谱(FTIR)和X射线衍射以及酶水解实验对不同粒径范围(300～450μm、125～150μm、97～105μm和330～420nm)的高粱秆进行分析。结果表明:随着粉碎程度增大,高粱秆中纤维素与酶有效接触点增多,但由于团聚现象发生,导致酶有效接触点增多并不与粒度减小成线性关系;粉碎还导致高粱秆结晶度降低,结品度从粒径范围300～450μm时的0.6105降低到330～420nm时的0.2397,并对结晶区晶型结构和高粱秆物质结构有影响,但是对高粱秆成分没有影响。这些都导致在后续的酶解糖化实验中还原糖浓度随酶解时间和粉碎程度的增大而增大,其中粒径范围330～420nm的高粱秆在酶解180h时仍保持较大的酶解转化率。
The sorghum stalks with different grain sizes (300 ~ 450μm, 125 ~ 150μm, 97 ~ 105μm and 330 ~ 420nm) were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction. The results showed that the effective contact point between cellulose and enzyme in sorghum stalks increased with the degree of comminution, but due to agglomeration, the increase of effective contact point did not lead to a linear relationship with the decrease of particle size; crushing also led to the formation of sorghum stalk Degree decreased, the degree of conjugation decreased from 0.6105 at 300 ~ 450μm to 0.2397 at 330 ~ 420nm, and had an effect on the crystalline structure of the crystalline zone and the material structure of sorghum stalk, but had no effect on the sorghum stalk composition. All these resulted in that the concentration of reducing sugar increased with the increase of enzymolysis time and degree of pulverization in the subsequent enzymolysis and saccharification experiments. Sorghum stalk with particle size of 330-420 nm remained a large enzymolysis for 180h Conversion rate.