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以普通小麦新601×雌性不育小麦XND126的F2群体作为育性调查以及基因标记群体.通过对育性基因的分析,确定在此组合中雌性不育基因由1对主效基因控制;结合混合分组分析法(Bulk Segregant Analysis,BSA),首次对小麦雌性不育基因进行了SSR分子标记,通过对一千对微卫星引物的筛选,确定微卫星引物cfd36标记与主效基因连锁,遗传距离为20.2cM.
F2 population of new wheat × Oryza sativa × Oryza sativa × Oryza sativa × Oryza sativa was used as a fertility survey and genetic marker population.Analysis of fertility genes showed that the female sterility gene was controlled by one pair of major genes in this combination. For the first time, SSR markers of female sterility genes were carried out by using Bulk Segregant Analysis (BSA). Through screening of one thousand pairs of microsatellite primers, it was confirmed that the microsatellite primer cfd36 was linked with the major gene and the genetic distance was 20.2cM.