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目的:通过建立表达不同高低水平miRNA-194的骨肉瘤细胞系,研究和探测miRNA-194对于骨肉瘤细胞转移特性的影响和作用。为进一步研究miRNA-194作为生物治疗的新靶点提供理论依据。方法:使用慢病毒技术,对骨肉瘤细胞进行转染和筛选,获得表达不同高低水平miRNA-194的骨肉瘤细胞系并进行分组。通过Transwell实验,划痕实验对miRNA-194在骨肉瘤中的作用进行探索。结果:1)慢病毒转染及筛选成功,获得表达不同高低水平miRNA-194的骨肉瘤细胞系;2)transwell迁移及侵袭实验中,miRNA-194过表达组的迁移(28.60±4.36)及侵袭(21.25±6.42)能力都显著的小于其余各组,相应的,miRNA-194沉默表达组(132.60±15.64;115.76±11.38)则高于其余各组。划痕实验结果显示,miRNA能够显著的抑制骨肉瘤细胞SOSP-9607的划痕愈合能力(P<0.01)。结论:miR-194能够对骨肉瘤细胞SOSP-9607的转移起到明确的抑制作用。MiRNA-194有望成为骨肉瘤转移与治疗的新靶点。
OBJECTIVE: To study the effect and role of miRNA-194 on metastasis of osteosarcoma cells by establishing osteosarcoma cell lines expressing different levels of miRNA-194. Provide theoretical basis for further study of miRNA-194 as a new target of biological therapy. METHODS: Osteosarcoma cells were transfected and screened using lentivirus technique, and osteosarcoma cell lines expressing different levels of miRNA-194 were obtained and grouped. Transwell experiments, scratch experiments to explore the role of miRNA-194 in osteosarcoma. Results: 1) The lentiviral transfection and screening were successful and osteosarcoma cell lines expressing different levels of miRNA-194 were obtained. 2) The migration and invasion of transwell induced miRNA-194 overexpression (28.60 ± 4.36) and invasion (21.25 ± 6.42) were significantly lower than the other groups, correspondingly, miRNA-194 silencing expression group (132.60 ± 15.64; 115.76 ± 11.38) was higher than the other groups. Scratch test results show that miRNA can significantly inhibit the healing ability of SOSP-9607 cells (P <0.01). Conclusion: miR-194 can obviously inhibit the metastasis of SOSP-9607 in osteosarcoma cells. MiRNA-194 is expected to become a new target for the metastasis and treatment of osteosarcoma.