论文部分内容阅读
DNA条形码技术是利用一段较短的DNA序列实现快速准确物种鉴定的工具,已成为近年来生物类群的研究热点,并逐步被引进到各个领域。在海洋生物群落生态学中,DNA条形码已被用于海洋生物系统分类、分子遗传多样性、种间亲缘关系、系统进化关系等研究;国外已有学者将其应用到海洋生物食性分析的研究中,国内相关文章较少。本文介绍了DNA条形码的由来、发展以及相关原理和基本实验步骤,并阐述了其优点以及局限性,分析了其在肉食性鱼类胃含物不可辨认种类鉴定中的应用价值,并展望其在未来分类学发展中的应用前景。
DNA barcoding technology is a tool to identify species quickly and accurately with a short DNA sequence. It has become a hot spot in the field of biological taxa in recent years and has been gradually introduced into various fields. In the marine community ecology, DNA barcodes have been used in marine biological system classification, molecular genetic diversity, interspecific relationship, phylogenetic relationships and other studies; some foreign scholars have applied it to the study of marine food habits , Less domestic related articles. This article introduced the origin, development, related principle and basic experimental steps of DNA barcodes, described the advantages and limitations of the DNA barcodes, and analyzed their application value in identifying the unidentifiable species of carnivorous fish stomach. Application prospects in the development of future taxonomy.