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目的通过建立兔小肠缺血再灌注模型，观察自由基在小肠缺血再灌注损伤时的变化。方法完全阻断和开放兔肠系膜上动脉，分别测定各时段血液中雨二醛（ＭＤＡ）含量及过氧化氢酶（ＣＡＴ）、超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ）的活性，观察肠组织形态学上的变化。结果ＭＤＡ含量随缺血和再灌注时间的延长而逐渐增高，且与小肠的损伤程度呈正相关；ＣＡＴ、ＳＯＤ、ＧＳＨ－ＰＸ活性随缺血和再灌注时间的延长而逐渐降低。结论小肠缺血后再灌注产生大量的自由基和ＭＤＡ，后者是造成组织损伤的主要原因之一。
Objective To establish a model of intestinal ischemia-reperfusion in rabbits and observe the changes of free radicals during intestinal ischemia-reperfusion injury. Methods The rabbit superior mesenteric artery was blocked and opened completely. The content of blood lipid and the contents of superoxide dismutase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) activity, observe the morphological changes of intestinal tissue. Results The content of MDA gradually increased with the prolongation of ischemia and reperfusion time, and had a positive correlation with the degree of intestinal damage. The activities of CAT, SOD and GSH-PX gradually decreased with the prolongation of ischemia and reperfusion time. Conclusion Intestinal ischemia and reperfusion produce a large number of free radicals and MDA, the latter is one of the main causes of tissue damage.