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应用二阶导数光谱法测定器械消毒液中苯扎溴胺（新洁尔灭）的含量。以２６５ｎｍ和２７６ｎｍ波长处的峰、谷之间的振幅值作为苯扎溴胺的定量信息，求得相关系数ｒ＝０．９９９８，平均回收率为１００．５％，变异系数为ＣＶ＝±１０．７２％。
Determination of Benzalkonium Bromide (Bromogeramine) in Instrument Disinfectant by Second Derivative Spectrophotometry. The correlation coefficient r = 0.9998 was obtained with the amplitude of peak and valley at 265nm and 276nm as the quantitative information of benzalkonium bromide. The average recovery was 100.5% and the coefficient of variation was CV = ± 10 .72%.