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对籼稻圭６３０和粳稻０２４２８及其Ｆ１通过花药培养获得的８１个ＤＨ系进行了ＲＦＬＰ分析，有２８个探针揭示了ＤＮＡ变异。８１个ＤＨ系不同程度地发生了变异，并具有以下特点：（１）ＤＮＡ变异类型包括限制性片段长度的变化、９ＮＡ片段的丢失以及ＤＮＡ序列的扩增；（２）变异发生在籼稻圭６３０供体片段中的频率高于粳稻０２４２８，表现出基因型差异；（３）染色体组中第３、８、９和１０染色体较少发生变异，在其它染色体上均存在易变异位点；（４）在染色体的一些区段，相邻的探针均揭示了ＤＮＡ变异，表明在染色体上存在ＤＮＡ易变异区域；（５）变异位点和变异类型具有特异性，在同一位点不同的ＤＨ系中发生相同的变异。
81 DH lines obtained from anther culture of indica Gui 630, japonica 02428 and their F1 were subjected to RFLP analysis, and 28 probes revealed DNA variation. Eighty-one DH strains mutated to varying degrees and had the following characteristics: (1) The types of DNA variation included the restriction fragment length change, the loss of 9NA fragment and the amplification of DNA sequence; (2) the variation occurred in indica rice Gui 630 The frequency of the donor fragment was higher than that of japonica rice 02428, showing the genotype differences. (3) The chromosomes 3, 8, 9 and 10 were less frequently mutated and all other chromosomes had variable loci. (4) ) In some segments of chromosomes, the adjacent probes reveal DNA variation, indicating that there are DNA-prone regions on chromosomes. (5) Mutation sites and mutation types are specific. DH strains with the same site The same variation occurred.