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在β-内啡肽的氨基末端证实甲硫脑啡肽序列存在被认为是前体一产物关系的一种征象。然而过去除腺脑垂体外,企图从含脑啡肽多的其他组织如纹状体、肾上腺髓质中证实有β-内啡肽存在,但却均未获成功。作者曾在肾上腺髓质提取物中析得一系列小的或较大的含脑啡肽的多肽,其分子量范围为500—22,000道尔顿,其脑啡肽样活性只在用胰蛋白酶消化之后才出现,胰蛋白酶消化液中有游离脑啡肽说明此五肽序列位于羧基末端;在6位与精或赖氨酸结合的六肽当脑啡肽序列存在于内部时亦曾
Confirmation of the methionine enkephalin sequence at the amino terminus of beta-endorphin is considered as a sign of precursor-product relationship. However, in the past, in addition to the adenohypophysis, it was attempted to confirm the presence of β-endorphin in other tissues containing enkephalin, such as the striatum and adrenal medulla, but none of them succeeded. The authors have analyzed in the adrenal medulla extract a series of smaller or larger enkephalin-containing peptides with molecular weights in the range of 500-22,000 daltons, enkephalin-like activity only after digestion with trypsin It appears that tryptic digestion of free enkephalin peptide sequence of this pentapeptide is located at the carboxyl terminus; 6 and the fine or lysine hexapeptide when enkephalin peptide sequence exists in the interior