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的检测HBV基因及其抗原在人原发性肝内胆管细胞癌中的表达,探讨人原发性肝内胆管细胞癌的发病因素及发病机制.方法用免疫组化技术检测HBV抗原,用原位分子杂交技术检测HBV基因.结果在20例肝内胆管细胞癌中,HBxAg,preS1,preS2,HBsAg和HBcAg阳性检出率分别为75%,40%,40%,10%和0%;在19例癌旁肝组织中,其阳性检出率分别为842%,479%,479%,316%和316%.在40例肝内胆管细胞癌中,HBVDNA,X基因,preS基因,S基因及C基因阳性检出率分别为775%,70%,475%,40%和425%;在33例癌旁肝组织中,其阳性检出率分别为879%,848%,636%,697%和667%.结论人原发性肝内胆管细胞癌的发生与HBV慢性感染有密切关系,特别是与HBVX基因及其蛋白在肝癌发病中起着更重要的作用
To detect the expression of HBV gene and its antigen in human primary intrahepatic cholangiocarcinoma and to investigate the pathogenetic factors and pathogenesis of human primary intrahepatic cholangiocellular carcinoma. Methods HBV antigen was detected by immunohistochemistry and HBV gene was detected by in situ hybridization. Results Among 20 cases of intrahepatic cholangiocarcinoma, the positive rates of HBxAg, preS1, preS2, HBsAg, and HBcAg were 75%, 40%, 40%, 10%, and 0%, respectively; In liver tissue, the positive detection rates were 84. 2%, 47.9%, 47.9%, 31.6% and 31.6%, respectively. In 40 cases of intrahepatic cholangiocarcinoma, the positive detection rates of HBV DNA, X gene, preS gene, S gene and C gene were 77.5%, 70%, 47.5%, 40% and 42 respectively. 5%; in 33 cases of adjacent liver tissue, the positive detection rate were 87.9%, 84.8%, 63.6%, 69.7% and 66.7%, respectively. Conclusion The occurrence of primary intrahepatic cholangiocarcinoma in human is closely related to the chronic infection of HBV, especially with HBVX gene and its protein plays an important role in the pathogenesis of liver cancer.