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目的:探讨二氢杨梅素(DMY)对湿性年龄相关性黄斑变性(AMD)的影响。方法:用532 nm激光对成年日本大耳白兔眼底进行光凝,创建AMD模型。光凝4周后,DMY(100 mg·kg~(-1)·d~(-1))灌胃给药,酶联免疫吸附法(ELISA)检测外周血中血管内皮生长因子(VEGF)和色素上皮衍生因子(PEDF)水平;实验结束时,苏木精-伊红染色法检测脉络膜新生血管(CNV);解剖实验动物,分别计算肝脏、肾脏和脾脏指数。结果:给药4周,模型组外周血VEGF降低幅度为63.3%,而模型对照组降低幅度为48.6%;给药8周,模型组外周血VEGF降低幅度为46.7%,模型对照组降低幅度为20.1%。给药4周,模型组外周血PEDF降低幅度为55.9%,而模型对照组降低幅度为58.6%;给药8周,模型组外周血PEDF降低幅度为12.1%,模型对照组降低幅度为53.1%。给药8周,模型对照组可见明显CNV,而模型给药组未见明显CNV;模型给药组肝脏指数和脾脏指数明显低于模型对照组(P=0.147和P=0.223),模型给药组肾脏指数与模型对照组没有明显差异(P=0.874)。结论:DMY具有一定的抑制湿性AMD模型日本大耳白兔CNV生长的药理作用,其作用机制可能与其下调VEGF的表达、上调PEDF表达和益气活血、健脾疏肝有关。
Objective: To investigate the effect of dihydromyricetin (DMY) on wet age-related macular degeneration (AMD). Methods: The photocoagulation of adult Japanese white rabbits was performed with 532 nm laser to create AMD model. After 4 weeks of photocoagulation, DMY (100 mg · kg -1 · d -1) was administered intragastrically, and the levels of vascular endothelial growth factor (VEGF) and total cholesterol in peripheral blood were measured by enzyme-linked immunosorbent assay (PEDF). At the end of the experiment, choroidal neovascularization (CNV) was detected by hematoxylin-eosin staining. The experimental animals were dissected to calculate the index of liver, kidney and spleen. Results: After 4 weeks of administration, the decrease of VEGF in the peripheral blood of the model group was 63.3%, while the decrease of the model control group was 48.6%. After 8 weeks of administration, the decrease of VEGF in the model group was 46.7% 20.1%. After 4 weeks of administration, the decrease of PEDF in peripheral blood of model group was 55.9%, while that in model control group was 58.6%. After 8 weeks of administration, the decrease of PEDF in peripheral blood of model group was 12.1% and that of model control group was 53.1% . After 8 weeks of administration, significant CNV was found in the model control group, but no significant CNV was found in the model group. The liver index and spleen index in the model group were significantly lower than those in the model control group (P = 0.147 and P = 0.223) There was no significant difference between the group of kidney index and the model control group (P = 0.874). CONCLUSION: DMY can inhibit the CNV growth of Japanese white rabbits in a wet AMD model. The mechanism may be related to its down-regulation of VEGF expression, up-regulation of PEDF expression, and invigorating qi and activating blood circulation and invigorating the spleen and liver.