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水稻(Oryza sativa)是我国最主要的粮食作物之一,其穗部形态直接影响着水稻产量和稻米品质。在秋光和七山占构建的重组自交系群体中发现了1个散穗突变体材料sp(spreading panicle),田间表现为穗部一次枝梗向外延伸,与穗轴夹角增大,且向四周散开,故暂命名为散穗突变体sp。与野生型相比,突变体sp穗重、每穗粒重、千粒重、粒宽以及粒厚均极显著减少,推测SP可能是1个参与调控穗部形态建成和颖花发育的基因。遗传分析表明,该性状受1个显性核基因控制。利用sp与02428构建的F2群体进行基因定位,将该基因定位在4号染色体长臂端,位于E3和RM17578之间的62.9 kb区域内。该结果将为SP基因的图位克隆和揭示其作用机理奠定基础。
Oryza sativa is one of the most important grain crops in China. Its ear morphology directly affects rice yield and rice quality. One panicle mutant, sp (spreading panicle), was found in the population of recombinant inbred lines constructed by Qiu-shuang and Qishan. In the field, the first branch of the panicle extended outward and the angle between it and the panicle increased Spread to the surrounding, so tentatively named spike mutant sp. Compared with the wild type, SP spike weight, grain weight per panicle, 1000-grain weight, grain width and grain thickness all decreased significantly. It was speculated that SP might be a gene involved in the regulation of spike morphogenesis and spike development. Genetic analysis showed that the trait was controlled by a dominant nuclear gene. The F2 population with sp and 02428 was used for gene mapping. The gene was mapped on the long arm of chromosome 4 in the region of 62.9 kb between E3 and RM17578. This result will lay the foundation for the map-based cloning of SP gene and reveal its mechanism of action.