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目的 研究髓过氧化物酶 (MPO)基因 46 3G→A单核苷酸多态与肺癌易感性的关系。方法 以PCR 单链构像多态 (SSCP)技术 ,分析了 314例肺癌患者和 32 0例正常对照者MPO基因启动子区第 46 3位核苷酸多态基因型分布 ,及其与肺癌风险的关系。结果 正常对照组中G和A等位基因频率分别为 85 .0 %和 15 .0 % ,而肺癌患者分别为 89.0 %和 11.0 %。携带MPO 46 3GG基因型的个体患肺癌风险比携带GA和AA基因型个体高 1.7倍 (校正的OR为 1.7;95 %CI为 1.2～ 2 .5 ) ,且此种风险增高只限于肺鳞癌 (OR为 2 .4;95 %CI为 1.4～ 3.9) ,而与肺腺癌无关 (OR为 1.3;95 %CI为0 .8～ 2 .1)。分层分析结果表明 ,与GA和AA基因型比较 ,GG基因型并不增加非吸烟者和累积吸烟量 <2 6包年者患肺癌的风险。但在累积吸烟量≥ 2 6包年的吸烟者中 ,GG基因型发生肺鳞癌的风险比GA和AA基因型高 3.3倍。结论 MPO 46 3A等位基因显著降低中国人发生吸烟相关性肺鳞癌的风险。
Objective To investigate the relationship between 46 3G → A polymorphism of myeloperoxidase (MPO) gene and lung cancer susceptibility. Methods The single nucleotide polymorphism (SSCP) of PCR was used to analyze the distribution of the 463th nucleotide polymorphism in the promoter region of MPO gene in 314 patients with lung cancer and 320 healthy controls and its association with lung cancer risk Relationship. Results The frequencies of G and A alleles in control group were 85.0% and 15.0% respectively, while those in lung cancer were 89.0% and 11.0% respectively. Individuals carrying the MPO 46 3GG genotype had a 1.7-fold higher risk of lung cancer than individuals carrying the GA and AA genotypes (adjusted OR 1.7; 95% CI 1.2-2.5) and this increased risk was limited to squamous cell carcinoma of the lung (OR 2.4; 95% CI 1.4-3.9), but not lung adenocarcinoma (OR 1.3; 95% CI 0.8-2.1). Stratification analysis showed that, compared with GA and AA genotypes, GG genotype did not increase the risk of lung cancer in non-smokers and cumulative smokers <2 6-pack years. However, among smokers who accumulated more than 26 months of smoking, the GG genotype had a 3.3-fold higher risk of lung squamous cell carcinoma than the GA and AA genotypes. Conclusion MPO 46 3A allele significantly reduces the risk of smoking-related lung squamous cell carcinoma in Chinese.