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探索一种简便灵敏的糖蛋白半定量法。在 ｐＨ ５．０，碳二亚胺（ＥＤＣ）催化下，辣根过氧化物酶（ＨＲＰ）与己二酰二肼反应生成肼化的ＨＲＰ（ＨＲＰ－ＨＺ），固相于硝酸纤维素膜上的糖蛋白（纤连蛋白或转铁蛋白）经过碘酸氧化其糖链中的糖基后生成的醛基可与ＨＲＰ—ＨＺ缩合，用ＨＲＰ的发包底物显色即可检出精蛋白的存在与否。结果：糖蛋白中，１０ ｎｇ的糖即可被检出。结论：与其他糖定量方法相比，该方法灵敏度高，方法简便，能够普遍应用。
Explore a simple and sensitive semi-quantitative method of glycoprotein. At pH 5.0, catalyzed by carbodiimide (EDC), horseradish peroxidase (HRP) reacts with adipic dihydrazide to form hydrazinated HRP (HRP-HZ), immobilized on a nitrocellulose membrane On the glycoprotein (fibronectin or transferrin) by iodine oxidation of sugar chains in the sugar chain after the aldehyde group can be generated with HRP-HZ condensation, HRP hairpin substrate color can be detected protamine The existence or not. Results: In the glycoprotein, 10 ng of sugar can be detected. Conclusion: Compared with other quantitative methods, this method has high sensitivity, simple method and universal application.