目的构建大鼠ＲＤＳ／ｐｅｒｉｐｈｅｒｉｎ真核表达载体ｐＡＬＴＥＲＲＤＳ，并观察它在ＣＯＳ１细胞中的表达。方法将全长度的ＲＤＳ／ｐｅｒｉｐｈｅｒｉｎｃＤＮＡ（１．５ｋｂ）插入到真核表达载体ｐＡＬＴＥＲＭＡＸ中，构建了ＲＤＳ真核表达载体ｐＡＬＴＥＲＲＤＳ。采用电穿孔技术将其转染ＣＯＳ１细胞，转染４８ｈ后，采用ＲＴＰＣＲ检测ＲＤＳ／ｐｅｒｉｐｈｅｒｉｎｃＤＮＡ的表达。结果采用ＲＴＰＣＲ方法可检测到ＲＤＳ／ｐｅｒｉｐｈｅｒｉｎｃＤＮＡ转录的ｍＲＮＡ。结论所构建的ＲＤＳ真核表达载体可在ＣＯＳ１细胞中表达。 Objective To construct the rat RDS / peripherin eukaryotic expression vector pALTER  RDS, and observe its expression in COS  1 cells. Methods The full-length RDS / peripherincDNA (1.5kb) was inserted into the eukaryotic expression vector pALTERMAX to construct the RDS eukaryotic expression vector pALTERRDS. The cells were transfected with COS1 cells by electroporation, and 48 hours after transfection, the expression of RDS / peripherincDNA was detected by RTPCR. Results RT-PCR method can detect RDS / peripherincDNA mRNA transcripts. Conclusion The constructed RDS eukaryotic expression vector can be expressed in COS 1 cells.