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研究了测定初感和再感采植株所结块茎内的马铃著卷叶病毒(PLRV)和马铃薯Y病毒(PVY)的必要条件。分析了用Rindile处理打破休眠期前的块茎。PLRV在休眠块茎中可准确地测出,而PVY只有在经Rindite处理并且在暗处于22℃高温的条件下保持2～3周的马铃薯块茎中才容易鉴定出来。感染块茎内的马铃著卷叶病毒的浓度脐部比顶端高,并在35天的试验期间内,其浓度保持恒定。然而发现马铃薯Y病毒在块茎顶端浓度大,并且在打破休眠期后迅速增加。在22℃贮藏期间,休眠块茎中的马铃薯Y病毒浓度下降。也讨论了ELISA方法用于块茎的鉴定。
The essential conditions for the determination of plaque-rye virus (PLRV) and potato virus Y (PVY) in tubers harvested from first and secondarily harvested plants were studied. Analysis of tubers treated with Rindile before breaking dormancy. PLRV can be accurately detected in dormant tubers, whereas PVY is only identified in potato tubers that have been treated with Rindite and held for two to three weeks in the dark at 22 ° C. The concentration of the leaf-rolling virus in the infected tuber with tuberosity was higher at the umbilicus than at the top and remained constant over the 35-day period. However, potato Y virus was found to be concentrated at the top of tubers and rapidly increased after breaking dormancy. During storage at 22 ° C, potato Y virus concentrations in dormant tubers decreased. The ELISA method is also discussed for the identification of tubers.