Objective: To study the correlation between CT dynamic enhanced scanning parameters and serum tumor markers before and after radiofrequency ablation in patients with lung cancer. Methods: 60 patients with advanced non-small cell lung cancer who were treated in this hospital between January 2015 and January 2017 were divided into control group (n=30) and observation group (n=30) by random number table method. Control group received conventional intravenous chemotherapy, and observation group received intravenous chemotherapy combined with radiofrequency ablation. The differences in CT dynamic enhanced scanning parameter levels and serum tumor marker contents were compared between the two groups of patients before and after treatment. Pearson test was used to evaluate the correlation between CT dynamic enhanced scanning parameters and serum tumor marker contents in patients with advanced non-small cell lung cancer. Results: Before treatment, the differences in CT dynamic enhanced scanning parameter levels, non-organ-specific tumor marker contents and vascular tumor marker contents were not statistically significant between the two groups of patients (P>0.05). After treatment, CT dynamic enhanced scanning parameters PH and perfusion value levels of observation group were lower than those of control group (P<0.05); serum non-organ specific tumor markers CA125, CA153, CEA and CYFRA21-1 contents of observation group were lower than those of control group; serum vascular tumor markers VEGF, Ang-2, HIF-1 and MMP-9 contents were lower than those of control group (P<0.05). Pearson test showed that CT dynamic enhanced scanning parameters PH and perfusion value levels in patients with non-small cell lung cancer were positively correlated with serum non-organ specific tumor marker and vascular tumor marker contents. Conclusion: Adjuvant radiofrequency ablation can significantly reduce the tumor malignancy of patients with advanced non-small cell lung cancer.