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过去的研究证明感染猴疟原虫的红细胞表膜上存有特异的疟疾抗原,但对人疟的了解尚少。为此,作者以人恶性疟原虫进行了研究。实验使用4种恶性疟原虫虫株,冈比亚的FCR3、泰国的T_2、巴西的IMTM25、乌干达的PLF,均按Trager等方法在体外进行培养,其中一批当原虫血症达到5%时,每3天加入新鲜的红细胞;另一批则连续培养2周以上不加入新鲜红细胞予以对比。试验阳性血清采自西非高疟区的11例冈比亚恶性疟病人,并以从未患疟的美洲人血清作对
Past studies have shown that there are specific malaria antigens on the erythrocyte surface membrane of P. falciparum, but little is known about human malaria. To this end, the authors studied human P. falciparum. Four Plasmodium falciparum isolates, FCR3 in the Gambia, T_2 in Thailand, IMTM25 in Brazil and PLF in Uganda were cultured in vitro according to the method of Trager et al. In one batch, when the parasitemia reached 5%, every 3 Day to add fresh red blood cells; the other batch is continuously cultured for more than two weeks without adding fresh red blood cells for comparison. The test positive sera were collected from 11 cases of P. gambiae in the high malaria region of West Africa and were administered with never-infected American serum