柑橘黄脉病是由柑橘黄化脉明病毒(Citrus yellow vein clearing virus,CYVCV)引起的一种新发病害,对柑橘产业尤其是柠檬产业危害较重。我国于2009年首次在云南省瑞丽市发现该病,四川、重庆、江西等省市也陆续发现并呈快速传播趋势。CYVCV检测方法包括指示植物鉴定、酶联免疫吸附法、直接组织点免疫法(宾羽等,2015)、RT-PCR(陈洪明等,2015)、逆转录环介导等温核酸扩增法(刘 Citrus yellow vein disease is a new disease caused by Citrus yellow vein clearing virus (CYVCV), which is harmful to citrus industry, especially lemon industry. China first discovered the disease in Ruili City, Yunnan Province in 2009, and Sichuan, Chongqing and Jiangxi provinces and municipalities also discovered one after another and showed a rapid spread trend. CYVCV detection methods include the identification of plant identification, enzyme-linked immunosorbent assay, direct tissue spot immunoassay (Bin Yu et al 2015), RT-PCR (Chen Hongming et al 2015), reverse transcription-mediated isothermal nucleic acid amplification