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WD-重复蛋白(WD-repeat protein)含有WD-40基序,又称为WD40重复蛋白,在植物对非生物逆境胁迫响应过程中具有重要的调控作用。红麻(Hibiscus cannabinus)是重要的工业纺织原料,是盐碱地土壤改良的先锋作物。本研究以红麻转录组中与WD40基因高度相似的Unigene为参考,设计引物,进行反转录PCR扩增,经Sanger测序获得基因Hc WD40-1(Gen Bank登录号:KX711617)的c DNA序列,生物信息学分析表明,该基因开放阅读框为1 356 bp,编码451个氨基酸,具有7个典型的WD40结构域。q RT-PCR分析表明,Hc WD40-1基因受盐和干旱胁迫诱导表达,同时受脱落酸(abscisic acid,ABA)和茉莉酸甲酯(methyl jasmonate,Me JA)的诱导。研究结果表明,Hc WD40-1基因是ABA和Me JA信号转导途径、盐和干旱胁迫应答途径的关键枢纽基因。该研究结果为红麻耐盐、抗旱的脱落酸信号调控网络和茉莉酸信号调控网络的研究提供基础资料。
WD-repeat protein, which contains the WD-40 motif, also known as the WD40 repeat protein, plays an important regulatory role in plant response to abiotic stress. Kenaf (Hibiscus cannabinus) is an important industrial textile raw material, is a pioneer crop salinization soil improvement. In this study, Uig gene with high homology to WD40 gene in kenaf transcriptome was used as a reference, primers were designed and used for reverse transcription PCR amplification. The c DNA sequence of Hc WD40-1 (Gen Bank accession number: KX711617) was obtained by Sanger sequencing Bioinformatics analysis showed that the open reading frame of this gene was 1 356 bp, encoding 451 amino acids with seven typical WD40 domains. q RT-PCR analysis showed that the Hc WD40-1 gene was induced by salt and drought stress and induced by abscisic acid (ABA) and methyl jasmonate (Me JA). The results show that the Hc WD40-1 gene is a key hinge gene of ABA and Me JA signal transduction pathway and salt and drought stress response pathway. The results provided the basic information for the study of kenaf acid-tolerant and drought-resistant abscisic acid signal regulation network and jasmonic acid signal regulation network.