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目的:探讨结直肠癌组织中长链非编码RNA母系印迹表达基因3(MEG3)的表达及其与肿瘤血管生成的关系。方法:用RT-PCR法检测42例结直肠癌患者癌组织与对应的癌旁组织标本,以及人结肠癌SW48细胞与人正常结肠NCM460细胞中MEG3表达,分析MEG3表达与患者临床病理因素的关系;免疫组化法检测上述组织标本中血管内皮生长因子(VEGF)的表达及血管密度,分析VEGF表达、血管密度与MEG3表达的相关性。结果:MEG3相对表达量在结直肠癌组织明显低于癌旁组织(0.12 vs.1.00,P=0.003),在SW48细胞中明显低于NCM460细胞(0.15 vs.1.00,P=0.007);MEG3的相对表达量与患者肿瘤位置、浸润深度、分化程度和淋巴结转移无明显关系(均P>0.05)。结直肠癌组织中,VEGF表达与血管密度均明显高于癌旁组织(0.13 vs.0.09;50.34 vs.36.57,均P<0.05);MEG3表达水平和VEGF表达水平及血管密度均呈负相关(r=-0.304、-0.342,均P<0.05)。VEGF是MEG3表达水平的独立影响因素(P=0.005)。结论:结直肠癌组织中MEG3表达降低,从而可能促进肿瘤组织VEGF表达量与血管生成。
OBJECTIVE: To investigate the expression of long-chain non-coding parental maternally imprinted gene 3 (MEG3) in colorectal cancer and its relationship with tumor angiogenesis. METHODS: The expressions of MEG3 in human colorectal cancer SW48 cells and human normal colon NCM460 cells were detected by RT-PCR method. The relationship between the expression of MEG3 and clinicopathological factors was analyzed. Immunohistochemical method was used to detect the expression of vascular endothelial growth factor (VEGF) and vascular density in the above tissue samples. The correlation between VEGF expression, vascular density and MEG3 expression was analyzed. Results: The relative expression of MEG3 in colorectal cancer tissue was significantly lower than that in adjacent tissue (0.12 vs. 1.00, P=0.003). It was significantly lower in SW48 cells than in NCM460 cells (0.15 vs. 1.00, P=0.007); MEG3 The relative expression level was not significantly related to the tumor location, depth of invasion, differentiation, and lymph node metastasis (all P>0.05). In colorectal cancer tissues, VEGF expression and vascular density were significantly higher than those in adjacent tissues (0.13 vs. 0.09; 50.34 vs. 36.57, all P<0.05); MEG3 expression was negatively correlated with VEGF expression and vascular density ( r=-0.304, -0.342, all P<0.05). VEGF was an independent factor in the expression of MEG3 (P=0.005). Conclusion: The expression of MEG3 in colorectal cancer tissue is decreased, which may promote the expression of VEGF and angiogenesis in tumor tissue.