论文部分内容阅读
目的 研究马鹿 (CervuselaphusLinnaeus)茸多肽的化学结构和促进细胞有丝分裂的生物活性。方法 用酸性水提取鹿茸多肽 ,多肽组分经过CM SepharoseFastFlow和SephadexG 5 0柱色谱分离后 ,再用反相HPLC (C6)纯化。结果 从马鹿茸得到一个单一多肽化合物。结合激光解析电离飞行时间质谱 ,N 端Edman降解和氨基酸分析结果表明 ,该多肽是由 32个氨基酸残基组成的直链多肽 ,分子量 32 16u ,主要含缬氨酸、丙氨酸、赖氨酸和甘氨酸 ,无半胱氨酸 ,其一级结构氨基酸排列顺序为 :N端 VLSAADKSNVKAAWGKVGGNAPAFGAEALLRM ,与已知蛋白序列同源性远小于 5 0 %。VAPP促进大鼠表皮细胞 (浓度 0 4 - 5 0mg·L-1)和家兔肋软骨 (浓度 10 - 5 0mg·L-1)细胞增殖。结论 鹿茸多肽是具有促进细胞增殖的新多肽
Objective To study the chemical structure and promote the biological activity of mitosis in Cervuselaphus Linnaeus. Methods The pilose antler polypeptide was extracted with acidic water. The peptide fractions were separated by CM Sepharose FastFlow and Sephadex G 50 column chromatography and purified by reverse phase HPLC (C6). Results A single peptide compound was obtained from pilose antler. Combining laser desorption ionization time-of-flight mass spectrometry, N-terminal Edman degradation and amino acid analysis showed that the polypeptide is a straight-chain polypeptide consisting of 32 amino acid residues and has a molecular weight of 32 16u, mainly containing proline, alanine, and lysine. And glycine, cysteine-free, its primary structure amino acid order: N-terminal VLSAADKSNVKAAWGKVGGNAPAFGAEALLRM, and the known protein sequence homology is far less than 50%. VAPP promoted the proliferation of rat epidermal cells (concentration 0.4-50 mg·L-1) and rabbit costal cartilage (10-50 mg·L-1). Conclusion Antler polypeptide is a novel polypeptide that promotes cell proliferation