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目的观察消散胶囊对结直肠癌模型小鼠白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)表达的影响。方法将接种直肠癌细胞LOVO 24 h后的BABL/c裸鼠随机分成5个组(每组6只),分别为模型对照组(0.9%NaCl溶液)、阳性对照组(复方斑蝥胶囊组)、消散胶囊低剂量组、消散胶囊中剂量组、消散胶囊高剂量组,分别给予相应干预。观察各组小鼠肿瘤瘤重,计算抑瘤率。采用HE染色法观察肿瘤组织病理变化情况,ELISA法检测血清中IL-2、TNF-α含量。结果 HE结果显示,与模型对照组相比,阳性对照组癌细胞出现大面积坏死,消散胶囊中、高剂量组的癌细胞出现大面积的坏死、凋亡,消散胶囊低剂量组癌细胞大体结构完整,几乎没有出现坏死的情况。与模型对照组比较,阳性对照组和消散胶囊高、中剂量组血清中的IL-2含量升高,差异有统计学意义(P<0.01);消散胶囊低剂量组差异无统计学意义(P>0.05)。与模型对照组比较,阳性对照组和消散胶囊高、中、低剂量组血清中TNF-α含量升高,差异有统计学意义(P<0.05,P<0.01)。结论消散胶囊可使小鼠结直肠癌组织出现坏死、凋亡,血清中IL-2和TNF-α升高。
Objective To observe the effect of Xiaosan Capsule on the expression of interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) in mice with colorectal cancer. Methods BABL / c nude mice, which were inoculated with LOVO for 24 h, were randomly divided into 5 groups (6 in each group), which were model control group (0.9% NaCl solution), positive control group Xiaowan capsule low-dose group, dissipating capsule medium dose group, dissipating capsule high dose group, were given the appropriate intervention. Observe the tumor weight of mice in each group and calculate the tumor inhibition rate. The pathological changes of tumor tissue were observed by HE staining. The levels of IL-2 and TNF-α in serum were detected by ELISA. Results HE results showed that compared with the model control group, the positive control group of large area of tumor necrosis, the capsule in the high dose group, large doses of cancer cells showed a large area of necrosis, apoptosis, dissipate capsules, low-dose group, the general structure of cancer cells Complete, almost no case of necrosis. Compared with the model control group, the levels of IL-2 in the serum of the positive control group and the dissipative capsule high and medium dose groups were significantly increased (P <0.01), and there was no significant difference in the low-dose Xiaowan capsule group (P > 0.05). Compared with the model control group, the levels of TNF-α in the serum of the positive control group and the dissipative capsule high, medium and low dose groups were significantly increased (P <0.05, P <0.01). Conclusion Digestion capsules can cause colorectal cancer tissue necrosis, apoptosis, serum IL-2 and TNF-α increased.