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S-adenosylmethionine (SAM) plays important role in trans-methyl reactions. Under the condition of drought (30% PEG), salinity (200 mmol · L-1 NaCl) and low temperature (4℃), total RNA was extracted from the leaf and the first strand of cDNA was synthesized with reverse transcription. S-adenosylmethionine synthetase gene (SAMS gene) was amplified by PCR with the first strand cDNA as template and a pair of primers which was based on constructed ESTs sequence. Full-length SAMS gene sequence was obtained by BLAST comparison. According to the analysis, completed sequence of SAMS gene was integrality. The sequence of the SAMS gene was 1 185 bp in length with an opening reading frame (ORF) encoding 394 amino acids. The cDNA sequence showed a significant homology to the SAM genes from Phaseolus lunatus (89%), Medicago sativa (85%). A prokaryotic expression vectors based on pET-32b had been constructed and prokaryotic expression was analyzed in order to lay a strong foundation for resist adversity function analysis through situation of genic expression analysis.
Under the condition of drought (30% PEG), salinity (200 mmol · L-1 NaCl) and low temperature (4 ° C), total RNA was extracted from the leaf and the first strand of cDNA was synthesized with reverse transcription. S-adenosylmethionine synthetase gene (SAMS gene) was amplified by PCR with the first strand cDNA as template and a pair of primers which was based on constructed ESTs sequence. Full-length SAMS The sequence of the SAMS gene was 1 185 bp in length with an opening reading frame (ORF) encoding 394 amino acids. The cDNA sequence was a significant homology to the SAM genes from Phaseolus lunatus (89%), Medicago sativa (85%). A prokaryotic expression vectors based on pET-32b had been constructed and prokaryotic expression was analyzed in order to lay a strong foundation for resist adv ersity function analysis through situation of genic expression analysis.