论文部分内容阅读
用功能大分子复合体表观靶体积幅射失活法，比较二醋吗啡（海洛因）成瘾者，非吸毒志愿者及成功戒毒３个月以上者，其红细胞阿片受体与Ｇ蛋白耦联状态的异同．结果表明，对照组，成瘾组和康复组的阿片μ－受体复合体的大小（表观分子量）依次为：（３４７±２）ｕ（ｎ＝１１）；（２２８±５）ｕ（ｎ＝１６）和（２７９±１１）ｕ（ｎ＝９）．成瘾组明显低于对照组（Ｐ＜０．０１）；值得注意的是康复组虽然明显低于对照组（Ｐ＜０．０１），但明显高于成瘾组（Ｐ＜０．０１）．上述３组的阿片δ－受体复合体的大小（表观分子量）则分别为：（３０８±４）ｕ（ｎ＝１１），（２２４±４）ｕ（ｎ＝１６）和（２９９±７）ｕ（ｎ＝９）．成瘾组明显低于对照组（Ｐ＜０．０１）；同样值得注意的是康复组明显高于成瘾组（Ｐ＜０．０１），而且非常接近对照组（Ｐ＞０．０５）．结果提示：（１）二醋吗啡成瘾时红细胞阿片μ－和δ－受体均可能与Ｇ蛋白发生解耦联现象；（２）这种现象在红细胞是可逆转的．
Using functional macromolecule complex apparent target volume radiation inactivation method, compared with those who were addicted to vinegar (heroin), non-addicted volunteers and those who were successfully treated for more than 3 months, the red blood cell opioid receptor was coupled to G protein Similarities and differences in status. The results showed that the size (apparent molecular weight) of the opioid μ-receptor complex in the control group, addiction group and rehabilitation group were (347 ± 2) u (n = 11), (228 ± 5) u = 16) and (279 ± 11) u (n = 9). It is noteworthy that although the rehabilitation group was significantly lower than the control group (P <0.01), but significantly higher than the addiction group (P <0.01) . The opioid δ-receptor complex sizes (apparent molecular weights) for the three groups were (308 ± 4) u (n = 11), (224 ± 4) u ) U (n = 9). It was also notable that the rehabilitation group was significantly higher than the addiction group (P <0.01) and very close to the control group (P> 0.05). The results suggest that: (1) μβ and δ-receptors of erythrocytes may be decoupled from G protein during the addiction of di-vine morphine; (2) this phenomenon is reversible in erythrocytes.