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目的:探讨内毒素全身注射所致的角膜改变。方法:使用抗单核细胞、巨噬细胞和MHC-Ⅱ类抗原阳性细胞的单克隆抗体,用标准的ABC方法于内毒素注射前、后制备的角膜平片上进行免疫组织化学染色。结果:发现在正常角膜中央区实质内有散在分布的巨噬细胞,越近角膜缘分布越密集,MHC-Ⅱ类抗原阳性细胞则仅存在于角膜缘;内毒素注射后,中央区角膜单核巨噬细胞增多,这些细胞于角膜实质内发生了一系列形态学改变,MHC-Ⅱ类抗原阳性细胞仅见于注射后早期的中央区角膜内皮面。结论:内毒素诱导的角膜中巨噬细胞的增多可能是机体应激状态下的重要防御机制,角膜实质中MHC-Ⅱ类抗原的缺如则对维持角膜局部免疫微环境的稳定性有重要意义。眼科学报1996;12:70—74。
Objective: To investigate corneal changes caused by systemic injection of endotoxin. Methods: Monoclonal antibodies against monocytes, macrophages and MHC class II antigen-positive cells were used for immunohistochemical staining on corneal plain films prepared before and after endotoxin injection using the standard ABC method. Results: The distribution of macrophages in the central cornea of normal cornea was more densely distributed, and the more dense distribution of MHC-Ⅱ antigen positive cells was in the corneal limbus. After injection of endotoxin, the central corneal mononuclear Macrophages increased, these cells in the cornea within a series of morphological changes, MHC-Ⅱ antigen-positive cells found only in the early post-injection central corneal endothelial. CONCLUSION: Endotoxin-induced increase of macrophages in the cornea may be an important defense mechanism under stress conditions. The absence of MHC-Ⅱ antigen in the corneal parenchyma is of great importance to maintain the stability of corneal local immune microenvironment . Journal of Ophthalmology 1996; 12: 70-74.