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目的:为中药质量药理学评价方法体系的建立提供正面依据。方法:采用高效液相检测五个批次穿心莲药材中穿心莲内酯和脱水穿心莲内酯的含量;采用二甲苯所致小鼠耳肿胀试验表征药材的抗炎作用;采用细菌脂多糖所致大鼠发热试验表征药材的解热作用;根据上述结果分析用化学和药效学方法评估穿心莲药材质量的相关性。结果:不同批次药材的穿心莲内酯和脱水穿心莲内酯含量存在差异,其总含量分别为3.92%、3.89%、4.61%、3.67%、3.41%;上述药材均存在一定的抗炎和解热作用,且指标成分含量最高组的药效作用最为明显。结论:采用化学或药效学方法均可对药材质量进行评价,而在药效成分与质控指标成分一致时,两种方法评价结论呈正相关。
Objective: To provide a positive basis for the establishment of the method of quality pharmacology evaluation of traditional Chinese medicine. Methods: The contents of andrographolide and dehydroandrographolide in five lots of Andrographis paniculata were determined by high performance liquid chromatography (HPLC). The anti-inflammatory effects of medicinal materials were characterized by xylene-induced ear swelling in mice. The fever test characterizes the antipyretic effect of medicinal materials. According to the above results, the correlation between the quality of Andrographis paniculata by chemical and pharmacodynamic methods was analyzed. Results: The contents of andrographolide and dehydroandrographolide in different batches of medicinal herbs were different, the total contents were 3.92%, 3.89%, 4.61%, 3.67% and 3.41% respectively. All of the above herbs had certain anti-inflammatory and antipyretic effects , And the index of the highest content of the group of the most obvious pharmacodynamic effects. Conclusion: The quality of medicinal materials can be evaluated by chemical or pharmacodynamic methods. However, when the components of the medicinal and quality control indexes are consistent, the conclusion of the two methods is positive.