论文部分内容阅读
目的 探讨 17β 雌二醇 (17β E2 )或醋酸甲羟孕酮 (MPA)对卵巢癌细胞株血管内皮细胞生长因子 (VEGF)mRNA表达的影响。方法 采用 10 -12 ,10 -11,10 -10 ,10 -9,10 -8mol/L 17β -E2 或10 -9,10 -8,10 -7,10 -6,10 -5mol/LMPA作用于卵巢癌细胞株SKOV3细胞后 ,用半定量逆转录聚合酶链反应 (RT PCR)检测SKOV3细胞VEGFmRNA水平的变化。结果 VEGF的PCR产物均以 6 0 6bp和4 74bp为主。随着 10 -12 ～ 10 -8mol/L 17β E2 浓度的增加 ,SKOV3细胞VEGFmRNA的表达呈递增趋势 ;10 -11,10 -10 ,10 -9,10 -8mol/L 17β E2 组与对照组比较 ,差异有显著性 (P <0 .0 5 )。随着 10 -9～ 10 -5mol/LMPA浓度的增加 ,SKOV3细胞VEGFmRNA的表达呈下降趋势 ,且 6 0 6bp的变化比 4 74bp明显 ,6 0 6bp可能对MPA更为敏感 ,MPA各组与对照组比较 ,差异有显著性 (P <0 .0 5 ) ;除 10 -8,10 -7mol/LMPA组间比较外 ,其余各实验组间比较 ,差异有显著性 (P <0 .0 5 )。结论 雌激素可上调SKOV3细胞的VEGFmRNA水平的表达 ;孕激素可下调SKOV3细胞VEGFmRNA水平的表达。
Objective To investigate the effect of 17β-E2 or MPA on the expression of vascular endothelial growth factor (VEGF) mRNA in ovarian cancer cell lines. Methods 10 -12, 10-11, 10-10, 10-9, 10-8mol / L 17β-E2 or 10-9,10-8,10-7,10-6,10-5 mol / LMPA The ovarian cancer cell line SKOV3 cells were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT PCR) VEGF SKOV3 cells in the level of changes. Results The PCR products of VEGF were mainly 6 0 6bp and 4 74 bp. The expression of VEGF mRNA in SKOV3 cells increased with the increase of the concentration of 10 -12 ~ 10 -8 mol / L 17β E2. Compared with the control group, the expression of VEGF mRNA in 10 -11,10-10,10-9,10 -8mol / L 17β E2 group , The difference was significant (P <0. 05). With the increase of 10 -9 ~ 10 -5 mol / L MPA, the expression of VEGF mRNA in SKOV3 cells showed a decreasing trend, and the change of 60 6bp was more obvious than that of 74 74 bp, and the value of 60 6bp was more sensitive to MPA. (P <0.05). Except 10 -8 and 10 -7 mol / L MPA groups, there was significant difference between the other experimental groups (P <0.05) . Conclusion Estrogen can up-regulate the expression of VEGF mRNA in SKOV3 cells. Progesterone can down-regulate the expression of VEGF mRNA in SKOV3 cells.