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本研究以还原型辅酶Ⅱ黄递酶组织化学法对大鼠胆道感染体内模型中肝细胞表达诱生型一氧化氮合酶（ｉｎｄｕｃｉｂｌｅｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ｉＮＯＳ）的情况进行了研究，以了解这种条件下肝细胞是否有ｉＮＯＳ的表达及其表达的规律。方法：Ｗｉｓｔａｒ大鼠３０只，随机分为５组，对照１组，实验４组每组６只动物。实验组分别于第１ｄ下午４点，第２ｄ下午４点，第３ｄ上午８点和下午２点手术。第３ｄ下午４点处死全部动物并取材。模型制备：细针穿刺胆总管注射标准致病菌（大肠杆菌ＡＴＣＣ－２５９２２，美国菌种保藏中心提供）０２５ｍＬ，菌液浓度为１×１０８／ｍＬ，不全结扎胆总管（注射菌液后于针上结扎胆总管，再退针）。取材与组化染色：所有动物均于同一部分用锐利刀片切取绿豆大小两块肝组织，制成冰冻切片，以还原型辅酶Ⅱ黄递酶组织化学法对切片进行染色。图像分析：采用ＭＰＩＡＳ－５００多媒体彩色病理图像分析系统。结果：对照组切片低倍镜（×１００）视野所见为正常肝细胞，未见阳性细胞。感染２ｈ组可在中央静脉周围见到环形排列的着浅蓝色的弱阳性细胞及少数着深蓝色的强阳性细胞。感染８ｈ组在中央静脉周围可见大量着深蓝色的强阳性细胞，以中央静脉为中?
In this study, we investigated the expression of inducible nitric oxide synthase (iNOS) in hepatocytes of rat model of biliary tract infection with reduced coenzyme-diaphorase histochemical method to understand the role of liver in this condition Whether cells have iNOS expression and the law of expression. Methods: Thirty Wistar rats were randomly divided into 5 groups, control group 1 and experimental group 4 with 6 animals in each group. The experimental group were at 4:00 pm on the first day, at 4:00 pm on the 2nd day, and operated on the 3rd day at 8:00 am and 2:00 pm respectively. All animals were killed and drawn at 3d on the 3d day. Model preparation: Standard needle pathways of E.coli ATCC-25922 (supplied by American Type Culture Collection) were injected into the common bile duct by fine needle aspiration, and the concentration of bacterial fluid was 1 × 108 / mL. The common bile duct was not ligated Ligation of the common bile duct, and then back needle). Specimens and histochemical staining: All animals were in the same part with a sharp blade cut green bean size two liver tissue, made of frozen sections, with reduced coenzyme diaphorase histochemical staining of sections. Image Analysis: MPIAS-500 multimedia color pathological image analysis system. Results: The control group of low magnification (× 100) visual field seen as normal liver cells, no positive cells. Infection 2h group around the central vein to see the circular arrangement of light blue weakly positive cells and a few dark blue strong positive cells. 8h infection in the central vein around the central vein visible a large number of dark blue strong positive cells to the central vein as?