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从 10 0 0多份苎麻种质资源中筛选出抗旱性较强和较弱的栽培品种各 6个 ,选用 2 5个随机引物 ,对其总 DNA进行随机扩增。有 12个引物扩增得到了稳定的 RAPD图谱 ;扩增出的片段分子量在 0 .6～ 5 .15 kb之间 ,条带数在 3～ 15条之间 ,共扩增出 90个条带 ;采用聚类法中的中间距离法 ,对 12个品种的相似系数聚类生成树状图谱 ,分为两类三组 ,揭示了供试品种的亲缘关系 ,但抗旱基因型被分在不同的类一组中
Six hundred cultivars of ramie germplasm with strong and weak drought resistance were selected from 10 0 0 germplasm resources. Randomly amplified twenty five random primers were selected. Twelve primers amplified a stable RAPD map; the molecular weight of the amplified fragment was between 0.6-6.5 kb and the number of bands was between 3-15. A total of 90 bands were amplified Using the middle distance method in the cluster method, the similarity coefficients of 12 cultivars were clustered into two groups and three groups to reveal the genetic relationship of the tested cultivars, but the drought-resistant genotypes were divided into different In a group