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目的探讨早产大鼠胎盘白细胞介素-6(IL-6)、白细胞介素-10(IL-10)水平与支气管肺发育不良(BPD)的相关性。方法选择定期受孕的Sprague Dawley(SD)大鼠,于孕第20天剖宫产取出胎鼠及胎盘,其胎盘组织内IL-6、IL-10均采用免疫组织化学法及Western blot法(免疫印迹法)进行检测。早产鼠出生后置于高氧中持续喂养,于高氧暴露第14天,取早产鼠肺用于组织切片,进行苏木精-伊红(HE)染色后,观察肺组织形态学结构及放射性肺泡计数,从而判断早产鼠是否发生了BPD。根据肺泡计数及肺泡结构分为BPD组和非BPD组,分析两组胎盘IL-6、IL-10水平与早产鼠发生BPD的关联性。结果 BPD组胎盘IL-6表达水平明显高于非BPD组,差异有统计学意义(P<0.05);非BPD组胎盘IL-10表达水平高于BPD组,差异有统计学意义(P<0.05)。结论 IL-6可能促进BPD的发生,IL-10可能减少BPD的发生。
Objective To investigate the correlation between placental interleukin-6 (IL-6), interleukin-10 (IL-10) and bronchopulmonary dysplasia (BPD) in preterm rats. Methods Sprague Dawley (SD) rats were enrolled on a regular basis. The fetus and placenta were removed by cesarean section on the 20th day of pregnancy. The contents of IL-6 and IL-10 in the placenta were determined by immunohistochemistry and Western blot Blotting method). The premature rats were placed in high oxygen after birth and were fed continuously. On the 14th day of hyperoxia exposure, the lungs of premature rats were used for tissue sections and stained with hematoxylin-eosin (HE). The morphological structure of lungs and radioactivity Alveolar count, to determine whether premature babies have BPD. According to the alveolar count and the alveolar structure, the patients were divided into BPD group and non-BPD group. The correlation between IL-6 and IL-10 levels and BPD in preterm rats was analyzed. Results The level of IL-6 in placenta of BPD group was significantly higher than that in non-BPD group (P <0.05), and the level of IL-10 in non-BPD group was higher than that of BPD group (P <0.05) ). Conclusion IL-6 may promote the occurrence of BPD, IL-10 may reduce the incidence of BPD.