应用筑巢式递转录／聚合酶链反应方法对３２例慢性粒细胞白血病患者的ｂｃｒ／ａｂｌｍＲＮＡ进行检测，发现３１例有融合ｂｃｒ／ａｂｌｍＲＮＡ表达。其中１９例表达为ｂ３ａ２型，９例表达为ｂ２ａ２型，３例同时表达为ｂ３ａ２、ｂ２ａ２型。慢性粒细胞白血病临床分期与ｂｃｒ／ａｂｌｍＲＮＡ表达类型有关，加速期和急变期，同时表达两种类型居多，即易发生选择性拼接，该法灵敏度高达１０－６水平。 The detection of bcr/abl mRNA in 32 patients with chronic myelogenous leukemia by nested transcription/polymerase chain reaction was performed. 31 cases were found to have bcr/abl mRNA expression. Among them, 19 cases were expressed as b3a2 type, 9 cases were expressed as b2a2 type, and 3 cases were simultaneously expressed as b3a2, b2a2 type. The clinical stage of chronic myelogenous leukemia is related to the type of bcr/abl mRNA expression. In accelerating and blast crisis, the two types are expressed at the same time, that is, selective splicing is prone to occur. The sensitivity of the method is as high as 10-6.