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目的采用PCR法扩增食源性金黄色葡萄球菌中肠毒素基因以了解该菌肠毒素基因携带情况,比较食物中毒和食品监测来源菌株中肠毒素基因检出率差异。方法合成sea、seb、sec、sed和see五种肠毒素基因特异性引物,用常规PCR方法扩增食物中毒和食品监测来源菌株中各自肠毒素基因,同时采用mini-VIDAS检测食物中毒来源菌株中肠毒素。结果 110株菌株中有30株检出肠毒素基因,检出率为27.3%,肠毒素基因阳性菌株均只检出1种肠毒素基因。其中来自2起食物中毒的14株菌株均检出seb型肠毒素和相关基因,检出率为100%。来源于食品监测样本的96株菌株中有16株检出肠毒素基因,检出率为16.7%,包括sea型4株、seb型2株、sec型4株、sed型6株。结论在宁波市食品监测中所分离的金黄色葡萄球菌所携带的肠毒素基因主要有sea、seb、sec和sed四型,而seb型肠毒素是引起金黄色葡萄球菌肠毒素所致食物中毒的主要因素。
Objective To amplify the enterotoxin gene of Staphylococcus aureus by PCR to understand the carriage of enterotoxigenic gene of this strain and to compare the detection rate of enterotoxigenic gene in food poisoning and foodstuff-derived strains. Methods Sertoli, seb, sec, sed and see five specific primers were synthesized and their respective enterotoxin genes were amplified by conventional PCR from food poisoning and foodstuff monitoring strains. Simultaneously, mini-VIDAS was used to detect food poisoning strains Enterotoxin. Results 30 strains out of 110 strains were detected enterotoxin gene, the detection rate was 27.3%, enterotoxin-positive strains were detected only one type of enterotoxin gene. Among them, seb enterotoxin and related genes were detected in 14 strains of food poisoning from two sources, the detection rate was 100%. Sixteen out of 96 strains of food samples were detected for the presence of enterotoxin genes. The detection rate was 16.7%, including 4 sea type, 2 seb type, 4 sec type and 6 sed type. Conclusion Staphylococcus aureus isolated from foodstuff monitoring in Ningbo City mainly contains four kinds of enterotoxin genes, ses, ses, sed and sed, and seb enterotoxin is caused by Staphylococcus aureus enterotoxin-induced food poisoning major factor.