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目的:探讨FLK-1单克隆抗体对小鼠脉络膜新生血管(choroidal neovascularization,CNV)的治疗作用。方法:采用激光击射的方法诱导成年C57BL/6小鼠CNV模型,术前及术后3,6,9d利用腹腔注射的方法给与实验组小鼠FLK-1单克隆抗体500μg,激光术后10d处死小鼠,行脉络膜灌流铺片及组织病理学检查观察CNV的发展,定量评价FLK-1单克隆抗体对于实验性CNV的治疗作用。结果:术后10d所有激光光斑均发展为实验性CNV,脉络膜灌流铺片及组织病理学检查结果一致表明实验组小鼠CNV的发展受到明显抑制(P<0.001)。结论:FLK-1单克隆抗体可以抑制实验性CNV的发展,提示拮抗FLK-1可能成为治疗CNV有效的生物学方法之一。
Objective: To investigate the therapeutic effect of FLK-1 monoclonal antibody on choroidal neovascularization (CNV) in mice. Methods: Adult C57BL / 6 mouse CNV model was induced by laser irradiation. The mice were injected intraperitoneally with 500μg of monoclonal antibody of FLK-1 at 3, 6 and 9 days after operation. The mice were sacrificed on day 10d, and the development of CNV was observed by choroidal perfusion and histopathological examination. The therapeutic effect of FLK-1 monoclonal antibody on experimental CNV was quantitatively evaluated. Results: All laser spots developed into experimental CNV at 10 days after operation. The results of choroidal perfusion and histopathological examination showed that the development of CNV in experimental group was significantly inhibited (P <0.001). Conclusion: FLK-1 monoclonal antibody can inhibit the development of experimental CNV, suggesting that antagonizing FLK-1 may be one of the biological methods for the treatment of CNV.