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本研究利用受体自显影方法探讨了孤啡肽 (nociceptin/orphaninFQ)在外周炎症和痛觉过敏中的调制作用。将完全弗氏佐剂 (CFA)注射到大鼠一侧后脚掌 2d、4d或 8d后 ,分别进行脊髓C4[3H]孤啡肽结合物的定量测定。放射自显影光密度测定分析表明 ,注射CFA 4d后的动物与对照组相比 ,两侧脊髓Ⅰ -Ⅱ层的中间和外侧区均有 [3H]孤啡肽结合物的明显增加 ;而X层中放射性结合物没有任何改变。生化研究证实 ,[3H]孤啡肽与大鼠脑片的特异性结合 [3H]孤啡肽受体的结合特征是一致的。以上结果提示 ,脊髓 [3H]孤啡肽受体在痛觉过敏期间发生了上调 ;该反应可能通过增强内源性镇痛机制以减弱CFA引起的痛觉过敏。
In this study, we investigated the modulation of nociceptin / orphaninFQ in peripheral inflammation and hyperalgesia using receptor autoradiography. Complete Freund’s adjuvant (CFA) was injected into the rat’s dorsum of the paw 2 d, 4 d or 8 d later, respectively, to determine the amount of C4 [3H] orphanin binding in spinal cord. The results of autoradiography showed that compared with the control group, the levels of [3H] orphanin binding protein in the medial and lateral regions of the spinal cord I-II on both sides of the spinal cord were significantly increased in the X-ray There is no change in the radioactive conjugate. Biochemical studies confirmed that [3H] orphanin peptide and rat brain slice specific binding [3H] orphanin peptide receptor binding characteristics are consistent. These results suggest that spinal cord [3H] orphaninol receptors are upregulated during hyperalgesia; this response may attenuate CFA-induced hyperalgesia by enhancing endogenous analgesic mechanisms.