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水稻作为我国重要农作物,其营养生长和生殖发育过程都受到严格的生物钟的控制,因此,生物钟基因的表达变化也是决定水稻产量和种子质量的主要决定因素之一。为了更全面系统的了解生物钟基因在水稻生长和发育过程中的功能,本研究采用生物信息学共表达方法,筛选和鉴定水稻和拟南芥中生物钟基因,并对比分析了这些生物钟基因在单双子叶模式植物中的可能功能。从水稻表达谱公共数据库中筛选与生物钟基因表达密切相关732个基因,并对筛选出的水稻生物钟相关基因进行表达特性、节律性、生物功能预测及其与拟南芥的对比分析,结果表明水稻和拟南芥的生物钟基因可能都具有在生物钟核心振荡器部分功能相对保守的特点。功能预测分析也还表明水稻生物钟基因参与了8类生物学过程,尤其在应激反应和代谢过程的方面具有明显富集性,从而间接佐证了水稻对于外界环境的响应及其代谢过程具有严格时间调控的分子机理。
Rice, as an important crop in China, is under the control of the strict biological clock in its vegetative and reproductive development. Therefore, changes in the expression of the circadian clock genes are also one of the major determinants of rice yield and seed quality. In order to understand the function of circadian clock gene in rice growth and development in a more comprehensive and systematic way, we used bioinformatics co-expression method to screen and identify the circadian clock gene in rice and Arabidopsis thaliana, and compared these genes in single- Possible functions in leaf pattern plants. A total of 732 genes, which are closely related to the gene expression of the circadian clock, were screened from the public database of rice expression profiles. The expression characteristics, rhythmicity, biological function of the selected genes related to the circadian clock were compared with that of Arabidopsis thaliana. And the circadian clock genes of Arabidopsis thaliana may both have relatively conservative functions in the part of the clock oscillator. Functional prediction analysis also showed that the rice circadian clock genes are involved in eight biological processes, especially in the aspects of stress response and metabolic processes, thus indirectly confirming that rice has a strict time response to the external environment and its metabolic process Regulatory molecular mechanism.