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本文建立了同时测定小柴胡汤正丁醇有效部位中甘草苷、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、人参皂苷Re和人参皂苷Rb1含量的HPLC-DAD-ELSD法。采用Diamonsil C18(2)色谱柱(4.6 mm×250 mm,5.0μm),以乙腈–0.05%甲酸水溶液为流动相,梯度洗脱,流速1 mL/min,二极管阵列检测器的检测波长为276 nm,蒸发光散射检测器的漂移管温度为110 oC,载气流速氮气2.1 L/min,柱温为30 oC。结果表明:7种有效成分在一定范围内线性关系良好。该方法简便、快速、重复性好,可用于同时测定小柴胡汤正丁醇有效部位中7种有效成分的含量。
A HPLC-DAD-ELSD method was developed for the simultaneous determination of the contents of n-butyl alcohol, baicalin, baicalin, baicalein, wogonin, ginsenoside Re and ginsenoside Rb1 in the active fraction of Xiaochaihu Tang n-butanol. A Diamonsil C18 (2) column (4.6 mm × 250 mm, 5.0 μm) was used with a mobile phase of acetonitrile-0.05% formic acid as mobile phase at a flow rate of 1 mL / min. The detection wavelength of the diode array detector was 276 nm , Evaporative light scattering detector drift tube temperature of 110 oC, carrier gas flow rate of nitrogen 2.1 L / min, the column temperature of 30 oC. The results showed that: the seven active ingredients in a certain range of good linear relationship. The method is simple, rapid and reproducible, and can be used for the simultaneous determination of seven active ingredients in the effective fraction of n-butanol in Xiao Chai Hu Tang.