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目的:探究天麻素(gastrodin or GAS)对炎性痛小鼠诱致的脊髓背角I层神经元自发放电的影响。方法:成年小鼠10只,一侧后足底皮下注射完全弗氏佐剂(complete Freund’s adjuvant,CFA)制备炎性痛模型后,于6、12、24、48、96、168 h分别观察机械性缩足反射阈值(paw withdrawal mechanical threshold,PWMT)和热刺激缩足反射潜伏期(paw withdrawal thermal latency,PWTL)的变化。选取出生14~18 d小鼠,按照上述相同方法建立炎性痛模型,24 h后制备脊髓薄片(300μm),孵育1 h后用全细胞膜片钳技术通过灌流系统给予GAS(300μmol/L),并探究其对炎性痛脊髓背角I层神经元自发放电的影响。结果:(1)在成年小鼠炎性痛模型中,CFA诱致的痛觉过敏可持续1~2周,在24 h达到高峰;(2)在出生14~18 d小鼠CFA注射24 h后,脊髓背角I层神经元出现显著的自发放电增强现象;(3)GAS可以显著抑制脊髓背角I层神经元自发放电的频率,对幅度无显著作用。结论:GAS对炎症导致的脊髓背角I层神经元自发放电增强具有显著的抑制作用,进而降低神经元的兴奋性,从而达到缓解炎性痛的效果。
AIM: To investigate the effect of gastrodin or GAS on spontaneous firing in I layer neurons of spinal dorsal horn induced by inflammatory pain in mice. Methods: Ten adult mice were injected with complete Freund’s adjuvant (CFA) subcutaneously on one side to prepare inflammatory pain model. The rats were observed at 6, 12, 24, 48, 96 and 168 hours respectively. Paw withdrawal mechanical threshold (PWMT) and thermal stimulation paw withdrawal thermal latency (PWTL). Spinal cord slices (300μm) were prepared 24 hours after the establishment of inflammatory pain model according to the same method as above, and the GAS (300μmol / L) was given by whole cell patch clamp technique after perfusion. And explored its effects on the spontaneous firing of I layer neurons in the spinal dorsal horn of inflammatory pain. Results: (1) In the adult mouse model of inflammatory pain, the CFA-induced hyperalgesia lasted for 1 to 2 weeks and peaked at 24 h. (2) At 24 h after CFA injection, (3) GAS could significantly inhibit the frequency of spontaneous firing of I layer neurons in spinal dorsal horn, but had no significant effect on the amplitude. CONCLUSION: GAS has a significant inhibitory effect on the enhancement of spontaneous firing of I neurons in spinal dorsal horn induced by inflammation, thereby reducing the excitability of neurons so as to relieve the inflammatory pain.