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目的观察日本血吸虫感染小鼠IL 17A、IL 23p19的表达,探讨IL 17、IL 23在免疫调控中的作用。方法建立日本血吸虫感染的小鼠模型,采用ELISA和RT PCR法检测小鼠感染后1、2、4、6、8及10周脾细胞培养液上清中IL 17A、IL 23p19蛋白水平和mRNA水平的表达。结果蛋白及mRNA表达结果显示,感染小鼠IL 17A与IL 23p19的变化趋势基本一致。感染小鼠IL 17A蛋白表达水平于感染后1周迅速升高,之后持续下降,8周时表达水平低于正常对照组;其mRNA表达也于感染后1周升高,第2周开始下降。而IL 23p19蛋白的表达水平于感染后1周升高,第2周到达高峰,第4周开始下降;其mRNA水平起先一直在正常范围波动,感染后4周开始升高,第6周达到峰值,之后逐渐下降。结论 IL 17和IL 23在日本血吸虫感染小鼠体内协同表达,并于感染早期呈现显著上升趋势,提示IL 17、IL 23参与了血吸虫感染极早期的免疫病理过程。
Objective To observe the expression of IL 17A and IL 23p19 in mice infected with Schistosoma japonicum and explore the role of IL 17 and IL 23 in immune regulation. Methods The mouse model of Schistosoma japonicum infection was established. The levels of IL-17A, IL-23p19 and the level of mRNA of spleen cells in supernatant of spleen cells were detected by ELISA and RT-PCR at 1, 2, 4, 6, 8 and 10 weeks after infection expression. Results The results of protein and mRNA expression showed that the changes of IL-17A and IL-23p19 in infected mice were basically the same. The level of IL-17A protein in infected mice increased rapidly at 1 week after infection, and then decreased continuously. The expression level of IL-17A in infected mice was lower than that of normal control group at the 8th week. The mRNA level of IL-17A increased at the first week and then decreased at the second week. However, the level of IL23p19 protein increased at 1 week after infection, peaked at 2 weeks and began to decline at 4 weeks. The mRNA level of IL23p19 began to fluctuate in the normal range at first, then increased at 4 weeks and peaked at 6th week , Then gradually declined. Conclusions IL 17 and IL 23 are synergistically expressed in mice infected with Schistosoma japonicum, and show a significant upward trend in the early stage of infection, suggesting that IL 17 and IL 23 are involved in the extremely early immunopathological process of schistosoma infection.