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英国药典规定透明质酸酶的分析是利用酶法降解透明质酸样品,而未被降解的透明质酸与血清可生成凝块,然后进行浊度测量。Whiteman(1973)用染料Alcian Blue 8GX加到透明质酸中生成沉淀,该沉淀洗涤后再溶解在40%双-异丁基-磺基琥珀酸酯溶液中(pH5.8)然后于603nm处测其吸收值。本文将上法加以改进,试验在带盖的塑料离心管(1.9ml)中进行。全部溶液都用含有0.05M氯化镁的0.05M醋酸钠缓冲溶液(pH5.0)制备。将含2～20酶
The British Pharmacopoeia states that the hyaluronidase assay uses enzymatic degradation of hyaluronic acid samples, whereas the non-degraded hyaluronic acid and serum yield clots, which are then measured for turbidity. Whiteman (1973) added a dye Alcian Blue 8GX to hyaluronic acid to generate a precipitate which was washed and then dissolved in 40% bis-isobutyl-sulfosuccinate solution (pH 5.8) and then measured at 603 nm Its absorption value. This article will be improved on the law, the test in a plastic centrifuge tube with lid (1.9ml) carried out. All solutions were prepared with 0.05 M sodium acetate buffer (pH 5.0) containing 0.05 M magnesium chloride. Will contain 2 ~ 20 enzyme