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从创伤弧菌(Vibrio vulnificus)中提取菌体脂多糖(lipopolysaccaride,LPS)和外膜蛋白(outer membrane protein,OMP)并制备福尔马林灭活全菌苗(FKC),腹腔注射接种黄姑鱼。分别在注射第0、7、14、21和28天后测定了受免鱼血清中凝集抗体效价、血清溶菌酶活性和血液白细胞吞噬活性,以及免疫28d后的相对免疫保护率。结果表明,3种抗原对黄姑鱼均有较强的免疫原性。免疫后,免疫组血清凝集抗体效价逐渐增高,第28天时最高;溶菌酶活性(LMZ)、白细胞吞噬活性(PP)和吞噬指数(PI)显著升高(P<0.01),第21天达到峰值,随后逐渐下降。各组之间比较表明,受免后7、14、21和28d,免疫组黄姑鱼凝集抗体效价、PP、PI和LMZ显著高于对照组(P<0.05),LPS和OMP组凝集抗体效价低于FKC组,LPS和OMP组的相对免疫保护率高于FKC组,各组间免疫保护率大小顺序为LPS组>OMP组>FKC组>对照组。
The bacterial lipopolysaccaride (LPS) and outer membrane protein (OMP) were extracted from Vibrio vulnificus, and the whole-cell vaccine of formalin-inactivated (FKC) was prepared. fish. The titers of agglutinating antibody, lysozyme activity and leukocyte phagocytosis activity of the immunized fish were measured at 0, 7, 14, 21 and 28 days after injection, respectively, and the relative immunoprotection rates after 28 days of immunization were determined. The results showed that the three antigens on the yellow croaker have strong immunogenicity. After immunization, the titer of serum agglutination antibody increased gradually on the 28th day, the lysozyme activity (LMZ), leukocyte phagocytic activity (PP) and phagocytic index (PI) increased significantly (P <0.01) Peak, then gradually decline. The comparison between the groups showed that the agglutination antibody titers, PP, PI and LMZ in the immunized group were significantly higher than those in the control group (P <0.05) at 7, 14, 21 and 28 days after immunization. Agglutination antibody The titer was lower than that of FKC group. The relative immunoprotection rates of LPS and OMP group were higher than those of FKC group. The order of immunization protection rate was LPS group> OMP group> FKC group> control group.