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采用大肠杆菌和根瘤菌接合的方法,将gusA标记基因分别导入了2株慢生型花生根瘤菌Spr3-5和Spr4-5中。结果表明,gusA基因在2株慢生型花生根瘤菌Spr3-5和Spr4-5中可以有效表达;对出发菌株和标记菌株的代时测定结果表明,二者之间没有显著差异;在标记菌株与出发菌株等量接种的前提下,比较了二者的竞争结瘤能力,结果表明,标记菌株形成的根瘤(蓝瘤)的占瘤率与出发菌株差异不显著;为了研究标记菌株与出发菌株的固氮有效性,测定了标记菌株与出发菌株各自共生植株的干重、全氮和叶绿素含量,结果表明,标记菌株与出发菌株的这3项指标之间没有显著差异。这说明利用gusA基因研究慢生根瘤菌的竞争结瘤能力是可行的。
The gusA marker gene was introduced into two strains of Bradyrhizobium sp. Spr3-5 and Spr4-5, respectively. The results showed that the gusA gene was efficiently expressed in two slow-growing strains of Rhizobium sp. Spr3-5 and Spr4-5. The generation-by-generation assay of the starting strain and the marker strain showed no significant difference between the two strains. The results showed that there was no significant difference between the tumor-bearing rate of the nodule (blue tumor) and the starting strain formed by the marker strains. In order to study the relationship between the marker strain and the starting strain , The dry weight, total nitrogen and chlorophyll content of the symbiotic plants of the marker strain and the original strain were measured. The results showed that there was no significant difference between the three indicators of the marker strain and the starting strain. This shows that it is feasible to study the competitive nodulation ability of Bradyrhizobia using gusA gene.