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应用显微分光光度计,并结合粘液组织化学方法观测了18例临床大肠癌和1例非癌大肠标本的正常肠上皮细胞、癌旁粘膜(移行粘膜)上皮细胞和癌细胞核DNA含量(石蜡切片,Feulgen染色),并初步探讨“移行粘膜”的意义。正常大肠粘液以硫酸粘液为主,可伴有少量的唾液酸粘液,但唾液酸粘液在大肠的不同肠段分布不同。核DNA含量测定表明,正常大肠上皮细胞核DNA含量(X=6.972)分布较集中,绝大多数细胞为近二倍体～近四倍体分布,几乎没有超四倍体
The nuclear DNA content of normal intestinal epithelial cells, paracancerous mucosa (transitional mucosa) epithelial cells and cancer cells in 18 cases of colorectal cancer and 1 case of non-cancerous large intestine specimens were observed using a microscopic spectrophotometer and combined with mucohistochemical methods (paraffin sections). Feulgen staining) and preliminary exploration of the significance of “transitional mucosa.” Normal mucus of the large intestine is dominated by sulfated mucus, which may be accompanied by a small amount of sialic acid mucus, but the distribution of sialic acid mucus is different in different intestinal segments of the large intestine. Nuclear DNA content analysis showed that the distribution of nuclear DNA (X=6.972) in normal colorectal epithelial cells was more concentrated, with the vast majority of cells being near-diploid to near-tetraploid, with almost no ultratetraploids.