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目的比较梅毒甲苯胺红不加热血清试验(TRUST)与梅毒螺旋体抗体胶体金诊断试剂两种试剂检测梅毒的特异性和敏感性。方法待测血清用含Reiter株螺旋体提取物吸收后,进行密螺旋体颗粒凝集试验(TPPA)检测,确认为梅毒阳性。再用甲苯胺红不加热血清试验和梅毒螺旋体抗体胶体金诊断试剂进行检测。结果梅毒螺旋体抗体胶体金诊断试剂敏感性97.5%,特异性为96.4%。TRUST试验敏感性为72.5%,特异性为85.7%。结论梅毒螺旋体抗体胶体金诊断试剂操作简便,反应时间短,便于临床推广应用。TRUST试验主要适合用于梅毒的筛查和治疗效果的监测。
OBJECTIVE: To compare the specificity and sensitivity of syphilis with TRUST and Treponema pallidum antibody colloidal gold diagnostic reagents. Methods The serum to be tested was absorbed by spirulina extract containing Reiter strain and tested for Treponema pallidum agglutination test (TPPA). Toluene red unheated serum test and Treponema pallidum antibody colloidal gold diagnostic reagents were tested. Results Treponema pallidum antibody colloidal gold diagnostic reagent sensitivity of 97.5%, specificity of 96.4%. TRUST test sensitivity was 72.5%, specificity was 85.7%. Conclusion Treponema pallidum antibody colloidal gold diagnostic reagent simple, short reaction time, easy to promote the clinical application. TRUST test is mainly suitable for the screening of syphilis and the monitoring of the therapeutic effect.