论文部分内容阅读
在室内用转Bt基因棉和亲本常规棉饲养甜菜夜蛾(Spodoptera exigua Hübner)幼虫,测定了不同取食时间后甜菜夜蛾4龄幼虫体内营养物质含量和消化酶、保护酶、解毒酶活力的变化.结果表明,分别取食Bt棉和常规棉,甜菜夜蛾幼虫体内的营养物质含量和消化酶、保护酶、解毒酶活力差异显著.与取食常规棉相应时间的个体相比,取食Bt棉1,6,24h后,幼虫体内的游离脂肪酸和葡萄糖含量显著提高;取食Bt棉1,4,6,24h后,幼虫体内胰蛋白酶和总超氧化物歧化酶的活力显著增高,脂肪酶、羧酸酯酶和乙酰胆碱酯酶活力则显著降低.取食相同品种棉花,幼虫体内营养物质含量和消化酶、保护酶、解毒酶活力受甜菜夜蛾危害时间的显著影响.取食Bt棉24h的幼虫,其体内游离脂肪酸和总氨基酸含量显著低于取食1h的个体;脂肪酶和胰蛋白酶活力显著低于取食1和4h的个体,但羧酸酯酶和乙酰胆碱酯酶酶活力显著高于取食1,4,6h的个体.棉花品种和甜菜夜蛾为害时间的交互作用可显著影响甜菜夜蛾体内脂肪酶、胰蛋白酶、乙酰胆碱酯酶和总超氧化物歧化酶的活力.通过测定甜菜夜蛾体内保护酶和解毒酶活力对Bt棉响应的时间动态,对于评价植食性昆虫在毒素蛋白持续选择压力下的生理反应和抗性变化具有重要参考意义.
In the laboratory, Spodoptera exigua Hübner larvae were fed with Bt cotton and parental cotton, and the contents of nutrients and the activities of digestive enzymes, protective enzymes and detoxification enzymes of 4th instar larvae of beet armyworm were determined after different feeding times The results showed that there were significant differences in the contents of nutrients and the activities of digestive enzymes, protective enzymes and detoxification enzymes in beet worms fed Bt cotton and conventional cotton, respectively.Compared with the individuals fed conventional cotton at the corresponding time, The content of free fatty acids and glucose in larvae significantly increased after 1, 6 and 24 h of Bt cotton treatment. The activities of trypsin and total superoxide dismutase The activities of enzymes, carboxylesterases and acetylcholinesterase decreased significantly.The content of nutrients in the larvae and the activities of digestive enzymes, protective enzymes and detoxification enzymes were significantly affected by the damage time of beet armyworm, The contents of free fatty acids and total amino acids in the larvae of 24 h were significantly lower than those of the individuals fed 1 h. The activities of lipase and trypsin in the larvae were significantly lower than those in the larvae of 1 and 4 h. However, carboxylesterase and acetylcholinesterase Vitality significantly higher than those fed 1, 4, 6h individuals.The interaction of cotton varieties and beet armyworm infestation time could significantly affect the activity of lipase, trypsin, acetylcholinesterase and total superoxide dismutase in beet armyworm The time response of Bt cotton to the activity of protective enzymes and detoxification enzymes in beet armyworm (WBT) is important for evaluating the physiological responses and resistance changes of herbivorous insects under the sustained selection pressure of toxin proteins.