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目的研究低分子量海带岩藻聚糖硫酸酯的制备和纯化技术,筛选抗血小板聚集活性的低分子量海带岩藻聚糖硫酸酯。方法以海带硫酸多糖为原料,采用自由基降解和强阴离子交换色谱技术,制备纯化出高硫酸根和高岩藻糖的低分子量岩藻聚糖硫酸酯;采用体外凝血酶诱导血小板聚集模型,筛选出抗血小板聚集活性的低分子量岩藻聚糖硫酸酯。结果强阴离子交换色谱能够有效地将复杂的低分子量海带岩藻聚糖硫酸酯分离纯化,得到4个纯度较高的高硫酸根、高岩藻糖的低分子量多糖,其岩藻糖含量超过85%~95%,硫酸根含量35%以上,分子量在20~30ku。体外抗血小板聚集试验结果证明,这4个高硫酸根高岩藻糖组分的抗血小板聚集活性最高,而低硫酸根组分含有较高的半乳糖、甘露糖和糖醛酸,其抗血小板活性非常低。结论采用自由基氧化降解和强阴离子交换色谱法,可获得抗血小板活性高的高硫酸根高岩藻糖的低分子量岩藻聚糖硫酸酯
Objective To study the preparation and purification of low molecular weight kelp fucoidan and screen low molecular weight kelp fucoidan with antiplatelet activity. Methods Kelp sulfate polysaccharide was used as raw material to prepare low molecular weight fucoidan sulfate with high sulfate and high fucose by free radical degradation and strong anion exchange chromatography. The thromboplastin-induced platelet aggregation model was screened out Anti-platelet aggregation activity of low molecular weight fucoidan sulfate. Results The strong anion exchange chromatography could effectively separate and purify the complex low molecular weight kelp fucoidan sulfate and obtain 4 high purity low sulfate and high fucose low molecular weight polysaccharides with fucose content over 85% ~ 95%, sulfate content above 35%, molecular weight 20 ~ 30ku. In vitro anti-platelet aggregation test results show that the four high-sulfate fucose components of the highest anti-platelet aggregation activity, while the low-sulfate components containing higher galactose, mannose and uronic acid, its anti-platelet activity very low. Conclusions The free radical oxidative degradation and strong anion exchange chromatography can be used to obtain low molecular weight fucoidan sulfate with high anti-platelet activity