论文部分内容阅读
以较抗天牛的Ｉ－６９杨为母本，以Ｉ－６３杨、欧洲黑杨和晚花杨２７２为父本，于１９８３年春进行人工控制授粉，组合编号分别为３４、３２和３７。经苗期选择选出优良单株营造比较林，经５年生无性系比较林和９年生无性系比较林测定，采用主成分分析、主成分遗传距离聚类分析和选择指数，选出速生、抗虫和材质较好的纤维材无性系３４－３０１和３４－２８６。这两个新选出的无性系（３４－３０１和３４－２８６），在９年生时与对照Ｉ－６９杨相比材积分别提高２４．９％和１６．６％，抗虫性明显提高（单株幼虫数分别为０．４和０．５，而Ｉ－６９杨为５．３８）；与３４－３１４相比材积分别提高１６．７％和８．９％。化学物质测定表明，抗虫性与树皮中酚类含量和丹宁含量呈正相关。分子生物学测定表明，杨树对云斑天牛的抗性受基因控制，找到了与抗虫基因连锁的ＲＡＰＤ分子标记。
Using I-69 as the female parent, the I-63 poplar, Populus nigra and the late poplar 272 as the male parent were artificially pollinated in the spring of 1983 with combinations numbered as 34, 32 and 37, respectively. Selected by the seedling selection of superior plant to create a comparative forest, the 5-year-old clonal climber and 9-year clonal comparison forest determination, using principal component analysis, principal components of genetic distance clustering analysis and selection index, select fast-growing, anti Insects and material better fiber clones 34-301 and 34-286. The two newly selected clones (34-301 and 34-286) increased their yields by 24.9% and 16.6% respectively at 9 years of age compared to control I-69, and significantly increased insect resistance The numbers of larvae per plant were 0.4 and 0.5, respectively, while that of I-69 was 5.38). The volume increased by 16.7% and 8.9% respectively compared with 34-314. Chemical analysis showed that there was a positive correlation between insect resistance and the content of bark phenols and tannin. Molecular biology tests showed that the resistance of poplar to P. aeruginosa was controlled by the gene, and the RAPD molecular marker linked to the insect resistance gene was found.