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目的:探讨京尼平(Genipin)对肾癌是否有治疗作用,该治疗作用是否与线粒体解偶联蛋白2(UCP2)相关。方法:将0、40、80和120μmol/L 4种浓度的Genipin溶液分别浸染肾癌细胞48 h,MTT法和流式细胞术检测细胞的增殖和凋亡情况;聚合酶链反应和ELISA方法检测UCP2的基因和蛋白表达;使用荧光探针检测细胞内钙离子和活性氧分子(ROS)含量。结果:Genipin在中、高剂量组能明显抑制肾癌细胞的增殖并促进细胞的凋亡,随着给药浓度的增加,细胞增值抑制作用及细胞凋亡率逐渐增高(P<0.005)。Genipin能抑制肾癌细胞中UCP2的表达,在中、高剂量给药组,UCP2的基因及蛋白的表达显著抑制(P<0.05)。Genipin能提高细胞内钙离子和ROS的含量,在中、高剂量给药组出现了显著提高(P<0.05)。结论:Genipin能明显抑制肾癌细胞的增殖,促进其凋亡,UCP2可能参与其作用机制。
OBJECTIVE: To investigate whether Genipin has a therapeutic effect on renal cell carcinoma and whether its therapeutic effect is related to mitochondrial uncoupling protein 2 (UCP2). Methods: The renal cell lines were treated with 0, 40, 80 and 120 μmol / L Genipin for 48 h respectively. MTT and flow cytometry were used to detect the proliferation and apoptosis of the cells. Polymerase chain reaction and ELISA UCP2 gene and protein expression; using fluorescent probes intracellular calcium and reactive oxygen species (ROS) content. Results: Genipin could significantly inhibit the proliferation of renal carcinoma cells and promote the apoptosis of cells in medium and high dose groups. With the increase of concentration, Genipin increased the inhibition of cell proliferation and the rate of apoptosis (P <0.005). Genipin can inhibit the expression of UCP2 in renal cancer cells. The expression of UCP2 gene and protein in the medium and high dose groups was significantly inhibited (P <0.05). Genipin increased intracellular calcium and ROS levels in the medium and high dose groups showed a significant increase (P <0.05). Conclusion: Genipin can significantly inhibit the proliferation and promote the apoptosis of renal cell carcinoma, and UCP2 may be involved in its mechanism of action.